Testing Framework

Mold and mycotoxin testing framework.

Different tests answer different questions. Start with what you’re trying to understand, then choose the testing method that fits.

Core Positioning

Start with the question,not the test.

An air test, a dust test, and a surface test may all be accurate — but they do not answer the same question. Identify what you’re trying to understand, then choose the method that matches.

The Question

Am I breathing it?

Recommended Protocol
AMEATest

Airborne Mycotoxin Environmental Analysis

The Question

What has accumulated over time?

Recommended Protocol
EMMATest

Environmental Mold & Mycotoxin Assessment

The Question

Is this surface contaminated?

Recommended Protocol
MESATest

Mycotoxin Environmental Surface Analysis

The Key Question

Am I breathingit?

Finding mold or mycotoxins somewhere in the home doesn’t automatically mean they’re being inhaled.

Step 1

Present in the home

A test confirms mold, spores, or mycotoxins exist somewhere — in dust, on a surface, in a material.

Detection
Step 2

Reaching the air you breathe

Exposure only happens when that contamination travels into occupied indoor air — through pathway, airflow, and time.

Exposure
Detection ≠ Exposure. Both matter — but they answer different questions.
Exposure Logic

The exposure framework.

Five questions that organize mold and mycotoxin findings into clear, comparable evidence.

Step 01

Hazard

What is it?

Mold, spores, DNA, or mycotoxins.

Step 02

Pathway

Are you breathing it?

Is the concern reaching occupied indoor air?

Step 03

Domain

Where was it found?

Air, dust, or surface.

Step 04

Timing

Is it happening now?

Current conditions or past accumulation.

Step 05

Verification

Was it resolved?

Did follow-up testing evaluate the original concern?

When these pieces align, results are easy to interpret. When they don’t, even accurate results can feel confusing.
Testing Domains

Air, dust, and surface answerdifferentquestions.

Air 01

What’s in the air I may be breathing?

Air testing evaluates what may be airborne during the sampling period — the most direct evidence for inhalation exposure questions.

Dust 02

What has settled over time?

Dust testing characterizes accumulated material — useful when the question is about history, not current air.

Surface 03

What’s on this specific surface?

Surface testing isolates contamination on a specific location, material, item, or HVAC component.

Definitions

Mold and mycotoxinsare not the same thing

Mold

A biological organism

Mold spores are biological particles mold uses to reproduce and spread. They may become airborne, settle into dust, or collect on surfaces.

How it's evaluated
  • Visual inspection
  • Moisture assessment
  • Spore trap testing
  • Mold DNA testing
  • EMMA dust testing
Mycotoxins

Chemical compounds

Mycotoxins are chemical compounds certain molds may produce under specific environmental conditions. They are not spores, mold DNA, or living mold.

How they're evaluated
  • AMEA™ air testing
  • EMMA dust testing
  • MESA™ surface testing
Quick Comparison

Compare every testing option.

Mold & Mycotoxin Test Comparison

Test Sample Measures Best question Best for
AMEA
Air exposure question
Air Mycotoxins Are mycotoxins present in the air I may be breathing? Breathing-zone mycotoxins
EMMA
Best initial dust screen
Dust Mold + mycotoxins What has accumulated in settled dust? Dust-based mold + mycotoxin history
MESA
Surface specific
Surface Mycotoxins Is this surface or item contaminated? Localized surface concern
Spore Trap
Air Mold spores What spores are airborne right now? Airborne mold spores
ERMI® / HERTSMI-2®
Dust Mold DNA What mold DNA accumulated in dust? Mold DNA profile
Interpreting Results

Why different tests can show different results

A positive dust result and a negative air result are not automatically contradictory — they’re answering different questions.

Positive dust / negative air

Material may have accumulated, but may not have been airborne during sampling.

Positive surface / negative dust

Contamination may be localized to a specific surface or location.

Positive biomarker / unclear source

Exposure may have occurred somewhere — the source is not identified by the biomarker alone.

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